Correlation between epigenetic alterations in the insulin growth factor-II gene and hepatocellular carcinoma / 中华肝脏病杂志
Zhi-zhen DONG; Deng-fu YAO; Wei WU; Li-wei QIU; Ning-hua YAO; Xiao-di YAN; Dan-dan YU; Jie CHEN.
Chinese Journal of Hepatology
; (12): 593-597, 2012.
Artículo
en Zh
| WPRIM | ID: wpr-296842
To investigate whether epigenetic alterations in the insulin-like growth factor-II (IGF-II) gene that cause differential transcription or expression are correlated with onset and severity of hepatocellular carcinoma (HCC). Patient-matched specimens of HCC, paracancerous, and non-cancerous tissues were collected from 40 primary liver cancer patients. Epigenetic alterations in the promoter (P3) sequence of the IGF-II gene were analyzed by methylation-specific PCR (MSP) and IGF-II transcription was measured by RT-PCR. IGF-II protein expression and clinicopathological features were assessed by immunohistochemistry and microscopic observation. The rate of IGF-II P3 methylation was significantly lower in HCC tissues (0%) than in paracancerous tissues (vs. 47.5%; x2 = 24.918, P less than 0.001) and non-cancerous tissues (vs. 100%; x2 = 80.000, P less than 0.001). IGF-II mRNA expression was significantly higher in HCC tissues (100%) than in paracancerous tissues (vs. 52.5%; x2 = 24.918, P less than 0.001) and non-cancerous tissues (vs. 0%; x2 = 80.000, P less than 0.001). IGF-II protein expression was significantly higher in HCC tissues (82.5%) than in paracancerous tissues (vs. 45.0%; x2 = 12.170, P less than 0.001) and non-cancerous tissues (vs. 0%; x2 = 56.170, P less than 0.001). IGF-II overexpression in HCC was significantly associated with degree of differentiation, extent of infiltrated serosa, size of tumor, and HBV-positive infection status. Epigenetic alterations in the IGF-II gene regulate its transcription and expression and are closely associated with HCC development and progression.
Asunto(s)
Adulto Humanos Persona de Mediana Edad Carcinoma Hepatocelular Genética Metabolismo Patología Islas de CpG Genética Metilación de ADN Epigénesis Genética Regulación Neoplásica de la Expresión Génica Inmunohistoquímica Factor II del Crecimiento Similar a la Insulina Genética Metabolismo Hígado Metabolismo Patología Neoplasias Hepáticas Genética Metabolismo Patología Reacción en Cadena de la Polimerasa Métodos Regiones Promotoras Genéticas ARN Mensajero Genética Metabolismo Transcripción Genética
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