Gaussia luciferase reporter assay for assessment of gene delivery systems in vivo / 中国医学科学杂志(英文版)
Feng CHEN; Zhen XU; Jiang LU; Xiang LÜ; Wen-li MU; Ya-jun WANG; De-pei LIU; Chih-chuan LIANG.
Chinese Medical Sciences Journal
; (4): 95-99, 2010.
Artículo
en Inglés
| WPRIM | ID: wpr-299451
<p><b>OBJECTIVE</b>To develop an alternative method for assessment of gene delivery systems in vivo.</p><p><b>METHODS</b>Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase (Gluc) expression cassette. After implantation of these cells into recipient mice, the expression of Gluc was detected in whole blood or plasma collected.</p><p><b>RESULTS</b>As little as 10 muL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer. And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice.</p><p><b>CONCLUSIONS</b>Gluc may be useful as an in vivo reporter for gene therapy researches, and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.</p>
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