Study on the prognostic value of hepatocyte growth factor and c-met for patients with hepatocellular carcinoma / 中华外科杂志
Fu-sheng WU; Shu-sen ZHENG; Ling-jiao WU; Wei DING; Zhi-min MA; Zhao-ming WANG; Li-song TENG; Wen-he ZHAO.
Chinese Journal of Surgery
; (12): 603-608, 2006.
Artículo
en Zh
| WPRIM | ID: wpr-300637
<p><b>OBJECTIVE</b>To analyze the prognostic value of hepatocyte growth factor (HGF) and c-met for patients with hepatocellular carcinoma (HCC) after hepatectomy.</p><p><b>METHODS</b>Twenty-five patients undergoing partial hepatectomy for HCC were studied. Serum HGF level was determined using ELISA kit before and after operation respectively. c-met protein and mRNA expression in cancerous and paracancerous tissues were detected by immunohistochemical and RT-PCR methods respectively. The correlations of clinical-pathologic parameters with the HGF level in serum and c-met expression in cancerous tissue were analyzed respectively.</p><p><b>RESULTS</b>HCC patients had a significantly higher concentration of serum HGF than normal controls and chronic hepatitis B respectively [(1.03 +/- 0.09) ng/ml vs (0.69 +/- 0.02) ng/ml and (0.74 +/- 0.09) ng/ml]. No significant difference in serum HGF was observed between HCC and cirrhosis patients with Child-Pugh score B/C [(1.03 +/- 0.09) ng/ml vs (1.04 +/- 0.11) ng/ml]. Serum HGF concentrations were positively correlated with tumor size (> 5 cm), node cirrhosis, portal vein tumor thrombi (PVTT) and preoperative alpha-fetoprotein (AFP) level (> or = 400 microg/L). After the resection of tumor, serum HGF concentration had a peak on the third postoperative day (POD), and then declined, but did not return to normal level on the tenth POD. From preoperative day to third POD, HGF concentration had a higher elevation in patients with major resection than with local resection. Moderately or strongly positive expression of c-met protein was observed in 21 cancerous regions (21/25), and only in 5 paracancerous regions. The intensive expression of c-met mRNA was 100% (25/25) detectable in the cancerous tissues, but only 24% (6/25) in the paracancerous tissues. The expression extent of c-met protein was correlated with portal vein tumor thrombi (PVTT). In paracancerous tissues, the expression of c-met protein was more intense in patients with cirrhosis than those without cirrhosis. The patients with recurrence or metastases after operation had a higher level of serum HGF and more intensive expression of c-met than other patients. No significant association was observed between HGF in serum and c-met expression in cancerous tissue.</p><p><b>CONCLUSIONS</b>The over-expression of HGF and its receptor c-met indicate an adverse prognosis for HCC patients. The sustained high level of serum HGF after hepatectomy may be a factor related to early tumor recurrence and metastasis.</p>
Asunto(s)
Adulto Anciano Femenino Humanos Masculino Persona de Mediana Edad Biomarcadores de Tumor Genética Metabolismo Carcinoma Hepatocelular Metabolismo Cirugía General Ensayo de Inmunoadsorción Enzimática Estudios de Seguimiento Hepatectomía Factor de Crecimiento de Hepatocito Sangre Neoplasias Hepáticas Metabolismo Cirugía General Pronóstico Proteínas Proto-Oncogénicas c-met Genética Metabolismo ARN Mensajero Genética Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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