<p><b>OBJECTIVE</b>To observe the intervention effect of Shugan Jianpi Formula (, SGJPF) on a
breast cancer mouse model with
depression and investigate the underlying mechanism of SGJPF in preventing the development of
breast cancer.</p><p><b>
METHODS</b>The
breast cancer model was induced by inoculation of
breast cancer cells, the
depression model was induced by chronic stress stimuli, and the
depression cancer model was established by combining the two factors. The
mice were divided into 7 groups normal control,
depression model,
tumor model,
depression tumor model, SGJPF,
chemotherapy, and SGJPF+
chemotherapy groups. The last 3 groups were
depression breast cancer mice and treated respectively with SGJPF,
chemotherapy drug gemcitabine (GEM), and SGJPF alongside GEM. The condition of the
mice was evaluated by the expression of
5-hydroxytryptamine in
hippocampus after the
sucrose water test and
open field test, weight change, and
survival time.
Tumor growth was monitored with in vivo imaging.
Flow cytometry was used to analyze the level of myeloid-derived
suppression cell (
MDSC) in the
mouse spleen,
T cell subsets, and the early
apoptosis of CD8T
cells.</p><p><b>RESULTS</b>The SGJPF+GEM group had the highest inhibition rate and the longest
survival time (P<0.01). The
MDSC level and the
apoptosis rate of CD8T
cells was the highest in the SGJPF+GEM group (P<0.05).</p><p><b>CONCLUSIONS</b>
Depressive disorders and
tumor growth could suppress the immune function of
mice to different degrees, and the microenvironment in late 4T1
inflammatory breast cancer may
play an important
role in the pathological process. SGJYF could regulate the immune microenvironment by reducing CD8T
lymphocyte apoptosis and
tumor cell activity, increasing immune
surveillance capability, and inhibiting
MDSC proliferation, thus prolonging the
survival time of
tumor-bearing
mice.</p>