Association of human chorionic gonadotropin level in embryo culture media with early embryo development / 南方医科大学学报
Haiying WANG; Renli ZHANG; Dong HAN; Caixia LIU; Jiajie CAI; Yanling BI; Anmin WEN; Song QUAN.
Journal of Southern Medical University
; (12): 1039-1047, 2014.
Artículo
en Zh
| WPRIM | ID: wpr-312642
<p><b>OBJECTIVE</b>To investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development.</p><p><b>METHODS</b>Spent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer (IVF-ET) cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed.</p><p><b>RESULTS</b>In the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample (93.8%) with a mean level of 0.85 ± 0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased (F=2.273, P=0.03), and decreased as the morphological grade of the embryo was lowered (F=3.900, P=0.02).</p><p><b>CONCLUSION</b>ELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.</p>
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