<p><b>OBJECTIVE</b>This study compared two
newborn Cytomegalovirus (CMV)
IgG antibody screening ELISA kits and evaluated the
detection effectiveness of Abnova kit.</p><p><b>
METHODS</b>CMV
IgG antibodies were detected by both SeraQuest and Abnova kits from dried
blood spot (DBS) samples of 488
newborn heel sticks. The
detection abilities of these two kits were compared in different sample
dilution concentrations. Relative
detection effectiveness of the Abnova kit was defined by statistical
method using the SeraQuest kit as a point of comparison.</p><p><b>RESULT</b>Compared to the SeraQuest
screening test kit, the Abnova kit revealed a
sensitivity of 98.9%,
specificity of 78.6%,
positive predictive value of 99.3%,
negative predictive value of 68.8%, and the coincidence rate for these two
screening test kits at 98.3%. The consistency check of both kits based on interpretation of the kappa
statistic was relatively good. For the Abnova kit, the "area under the
ROC curve" was 0.887, which indicates moderate accuracy.</p><p><b>CONCLUSION</b>Abnova kit can be applied to
newborn screening for
congenital CMV infections. However, repeating the test for ambiguous results is suggested to increase the
specificity and
negative predictive value.</p>