To develop a specific, rapid, and convenient
immunochromatography assay (ICA) to detect the
rabies antibody in clinical sample from immuned
dogs by
rabies vaccine.
Colloidal gold particles labeled with purified
rabies virus (CVS11) were used as the detector
reagent. The
staphylococcal protein A (SPA) and pured
rabbit anti-
rabies virus IgG were blotted on the test and control regions of
nitrocellulose membrane. Then the strip was assembled with sample pad, absorbing pad, and dorsal shield. The assay samples (261
dog's
serum) were collected from Wildlife
Rabies Disease Diagnostic
Laboratories of Ministry of
Agriculture in
China, Institute of
Military Veterinary,
Academy of
Military Medical
Sciences and other six provinces, including
rabies virus positive and negative
serum. The performance of the strip was compared to fluorescent antibody
virus neutralization test. The
neutralizing antibody titer could be detected above 0.5 IU. The strip did not change of performance when stored at room
temperature for 12 months. It may offer reference of
neutralizing antibody titer level after
dogs immuned
rabies vaccine and determin whether the
dogs need to be immuned again.