Effects of Nanofiber Alignment and Strain Direction on Cellular Activities of Human ACL Fibroblasts / 대한정형외과연구학회지
Chang-Hun LEE; Ho-Joon SHIN; In-Hee CHO; Young-Mi KANG; In-Ae KIM; Jung-Woog SHIN.
Journal of Korean Orthopaedic Research Society
; : 1-12, 2004.
Artículo
en Ko
| WPRIM | ID: wpr-32843
PURPOSE:
The effects of fiber alignment and direction of mechanical strain on the ECM generation of human ACL fibroblast were assessed. MATERIALS ANDMETHODS:
The aligned nanofiber was fabricated using electrospinning with a rotating target. The amounts of collagen on aligned and randomly oriented structures were compared. To evaluate the effect of strain direction, 5% uniaxial strain (0.2 Hz) was applied to fibroblasts seeded on parallel aligned, vertically aligned to the strain direction, and randomly oriented nanofiber sheets. The amounts of collagen produced were measured 2 days after halting the strain application.RESULTS:
The fibroblasts on the aligned nanofiber were spindle-shaped and oriented in the direction of the fibers. Significantly more collagen (22.5+/-2.7 ug/ngDNA) was synthesized on the aligned nanofiber than the randomly oriented (14.5+/-3.2 ug/ngDNA). And the amounts of collagen produced were increased by 150% and 50% approximately with the longitudinal and perpendicular cyclic strain, respectively.CONCLUSION:
The aligned nanofiber scaffold used in this study constitutes a promising base material for tissue-engineered ligament in that it provides a more biomimetic structure, including the preferable mechanical environment.
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WPRO
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