<p><b>OBJECTIVE</b>To investigate the effect of IL-24 expression on the growth of gliomacells.</p><p><b>METHODS</b>The IL-24 gene was transfected into ratglioma C6 cells with a retroviral vector. The expression of IL-24 in C6/IL-24 gliomacells was confirmed by RT-PCR. MTT assay and flow cytometry were used to study tumorcell proliferationin vitro. Tumorigenicity in vivo was studied in inbred SD malerats by the growth of intracerebrally inoculated tumor.</p><p><b>RESULTS</b>It was confirmed by RT-PCR that the exogenous IL-24 gene expressed in C6/IL-24 cell. The C6/IL-24 cell proliferationin vitro and tumorigenicity in vivo were both inhibited compared with its parental C6 cell.</p><p><b>CONCLUSION</b>IL-24 expression in gliomacells somehow inhibits their growthin vitro and in vivo.</p>