<p><b>OBJECTIVE</b>To study the effects of the generation 4 polyamidoamine/
vascular endothelial growth factor antisense
oligodeoxynucleotide (G4PAMAM/VEGFASODN) compound on the expressions of
vascular endothelial growth factor (
VEGF ) and its
mRNA of
breast cancer cells and on the inhibition of
vascular endothelial cells .</p><p><b>
METHODS </b>We examined the morphology of G4PAMAM/VEGFASODN compound and its
pH stability,
in vitro transfection efficiency and
toxicity , and the expressions of
VEGF and its
mRNA . Methyl thiazolyl tetrazolium assay was used to detect the inhibitory function of the compound on
vascular endothelial cells .</p><p><b>RESULTS</b>The compound was about 10 nm in diameter and was homogeneously netlike. From
pH 5 to 10, it showed quite a buffered
ability . The 48-h
transfection rate in the
charge ratio of 140 was 98.76%, significantly higher than that of the
liposome group (P<0.05). None of the
transfection products showed obvious
toxicity on the
cells . The expressions of both
VEGF protein and its
mRNA after G4PAMAM/VEGFASODN
transfection decreased markedly.</p><p><b>CONCLUSION</b>With a low
toxicity , high
safety , and high
transfection rate, G4PAMAM/VEGFASODN could be a promising
gene vector. Specifically, it inhibits
VEGF gene expression efficiently, laying a basis for further in vivo
animal studies.</p>