Determination of acetylharpagide in Ajuga decumbens by HPLC / 中国中药杂志
Dang-Kun YUE; Guang-Zhi DING; Wei-Hao WANG; Zhi-Min WANG; Jian-Rong LI; Yan TONG.
China Journal of Chinese Materia Medica
; (24): 289-290, 2006.
Artículo
en Zh
| WPRIM | ID: wpr-350958
<p><b>OBJECTIVE</b>To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.</p><p><b>METHOD</b>The chromatographic conditions were as follows a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (1585), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.</p><p><b>RESULT</b>Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.</p><p><b>CONCLUSION</b>The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.</p>
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