<p><b>OBJECTIVE</b>To study the flavonoid constituents in fresh herb of Houttuynia cordata.</p><p><b>METHOD</b>Various column packing materials including Diaion HP - 20, Sephadex LH - 20, ODS and silica gel were employed for the isolation and purification of compounds from H. cordata. The structures of the compounds were identified by physiochemical properties and spectral analysis.</p><p><b>RESULT</b>Five compounds were isolated, and their structures were identified as quercetin-3-O-beta-D-galactoside-7-O-beta-D-glucoside (1), kaempferol-3-O-alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranoside (2), quercitrin (3), hyperin (4), quercetin 3-O-alpha-L-rhamnopyranosyl-7-O-beta-D-glucopyranoside (5).</p><p><b>CONCLUSION</b>Compounds 1, 2 and 5 were separated from H. cordata for the first time.</p>