Scoring System for Detecting Spurious Hemolysis in Anticoagulated Blood Specimens
Gilsung YOO; Juwon KIM; Young UH; Kwang-Ro YOON; Soon-Deok PARK; Kap-Jun YOON.
Annals of Laboratory Medicine
; : 341-347, 2015.
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en Inglés
| WPRIM | ID: wpr-36804
BACKGROUND:
The identification of in vitro hemolysis (IVH) using a hematology analyzer is challenging because centrifugation of the specimens cannot be performed for cell counts. In the present study, we aimed to develop a scoring system to help identify the presence of hemolysis in anticoagulated blood specimens.METHODS:
Thirty-seven potassium EDTA anticoagulated blood specimens were obtained, and each specimen was divided into 3 aliquots (A, B, and C). Aliquots B and C were mechanically hemolyzed by aspirating 2 and 5 times, respectively, using a 27-gauge needle and then tested; aliquot A was analyzed immediately without any hemolysis. After the cells were counted, aliquots B and C were centrifuged and the supernatants were tested for the hemolytic index and lactate dehydrogenase levels.RESULTS:
The 4 hematologic parameters were selected and scored from 0 to 3 as follows or =38.5 for mean cell hemoglobin concentration (MCHC, g/dL); or =0.04 for red blood cell ghosts (10(12)/L); or =1.31 for difference value (g/dL) of measured hemoglobin and calculated hemoglobin; and or =3.35 for difference value (g/dL) of MCHC and cell hemoglobin concentration mean. The hemolysis score was calculated by adding all the scores from the 4 parameters. At the cutoff hemolysis score of 3, the IVH of aliquots B and C were detected as 64.9% and 91.9%, respectively.CONCLUSIONS:
The scoring system might provide effective screening for detecting spurious IVH.
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