Objective To investigate the
clinical significance of high mobility group box-1
protein (
HMGB1) in chronic,severe
hepatitis B patients and
liver cirrhosis.
Methods The contents of
HMGB1 was measured by
enzyme-linked
immunoassay (
ELISA) in the
patients (include 40
patients with
chronic hepatitis B and 18 severe
hepatitis B patients and 18
liver cirrhosis patients) and 20
health controls.The levels of relative biochemical
indicators,
prothrombin time activity percentage (PTA) and the hepatic
fibrosis were determined by biochemical
methods.Results The contents of
HMGB1 was significantly increased in severe
hepatitis patients in comparison with that in
chronic hepatitis B patients(P <0.01).The contents of
HMGB1 in the
chronic hepatitis were significantly increased in
health controls(P <0.01).The contents of
HMGB1 had significant difference among the
liver cirrhosis patients,the severe
hepatitis patients and the
chronic hepatitis(P <0.01).There was positive correlation among the contents of
HMGB1 and total
bilirubin (TBIL),the proportionality of
aspartate aminotransferase and
alanine aminotransferase (AST/ALT),
hyaluronic acid (HA) and
procollagen Ⅲ N-terminal
peptide (P Ⅲ NP) (r = 0.865,0.646,0.783,0.662,P < 0.01).There was negative correlation among the contents of
HMGB1 and
prothrombin time activity percentage (PTA) and
albumin (ALB) (r =-0.915,-0.852,P <0.01).Conclusion The contents of
serum HMGB1 were closely associated with
disease severity in
chronic hepatitis B patients.
HMGB1 was an index to auxiliary
diagnosis hepatic
fibrosis.