Objective To study the
clinical significance of urinary hTERT in
diagnosis and follow up of
transitional cell carcinoma of
urinary bladder.
Methods Semi-quantitative RT-PCR was used to detected the expression of hTERT
mRNA expression in 42 cases with
bladder transitional
carcinoma and 40
patients without
carcinoma of
bladder.Regular
urine cytology results were compared with the expression of hTERT.Following-up observision of the changes of hTERT
mRNA expression and its relation with recurrency in 36 cases of
transitional cell carcinoma of
urinary bladder was conducted.Results 30 0f 42 cases with
bladder transcriptional
carcinoma were found positive expression of hTERT.but only 3 in the
control group of 40 cases presented the positive expression of hTERT.The overall
sensitivity and specificity for hTERT were 71.43%and 92.50%.The sensitivities for hTERT were 50.00%,73.68%and 90.91%respectively in G1-G3
tumor.The
urine hTERT expression level significantly increased with the
tumor grade and clinical staging.The
sensitivity and the
specificity of urinary
cytology were 19.05%and 100%.Compared with hTERT.the
cytology had lower
sensitivity and no difference in
specificity.The
recurrence of the
tumor was found in 6
patients.8-16 weeks after positive expression of hTERT,
cystoscope confirmed
recurrence of
bladder carcinoma.Conclusions hTERT is a
tumor marker of transcriptional
carcinoma of
bladder.because of its relatively high
sensitivity and specificity.
Detection of hTERT in
urine sediment is superior to traditional
cytology.The
urine hTERT rate significantly increases with the
tumor grade and the clinical stage.hTERT can indicate
recurrence of
bladder carcinoma.so it is valuable to the
monitoring of
recurrence of transcriptional
cell carcinoma of
bladder.