Objective To investigate the preventive effect of
low molecular weight heparin or
tranexamic acid in the
rabbits with hypercoagulation induced by impact
injury and
intravenous infusion of the
endotoxin.
Methods Combination of impact
injury and
endotoxin was created by
strike on
rabbit chest with type BIM-Ⅱ
biological impact machine and
injection of
endotoxin via
vein.Thirty-two
male New Zealand rabbits were divided into four groups in a completely random design as followsimpact
injury combined with
endotoxin without
treatment group(
control group),
treatment with
low molecular weight heparin(L group),
treatment with
tranexamic acid(TA group),and
treatment with
low molecular weight heparin and tranexamie
acid(L+TA group).The changes of
interleukin-1β(
IL-1β)and
interleukin-6(
IL-6)were observed by using the
enzyme-linked immunosorbent assay;changes of
antithrombin-Ⅲ(AT-Ⅲ)activity,
tissue factor(TF)and
tissue factor pathway inhibitors(TFPI)were detected by the
chromogenic substrate method chromozym Pthe content of
fibrinogen was observed by coagulation analyzer.The
lung and
kidney were removed from the experimental
animals at 24 hours post impact
injury forhistopathologic
observation. Results In the
control group,the activity of AT-Ⅲ was decreased obviously.while the content of
fibrinogen and the activity of TF and TFPl were increased,with formation of the
thrombosis in the
kidney after
injection of PLS.Compared with
control group,the activity of TF and the content of
IL-1β and
IL-6 were reduced but the TFPI was increased in the L group,which resulted in a decrease of TF/TFPI ratio.with decrease of formation of the micro-
thrombosis in the organs.The content of
IL-1β and
IL-6 in the TA group were reduced,which was still higher than that in tIle L group.In the TA group.the activity of IF and TFPI Was lower than that in the L group and
control group,with higher TF/TFPI ratio than the L group.The frequency of
thrombosis increased in
pulmonary artery and other important organs.The content of
IL-1β and
IL-6 in the L+TA group wag lower than that in the L group.In the L+TA group.the activity ofTF was lower than that inthe L group and
control group,but the activity of TFPI was higher than that in the L group and
control group.with lower TF/TFPI ratio than the L group.There found no increase of
thrombus in the pulmonary arterial and other important organs. Conclusions Besides anticoagulation function,the
low molecular weight heparin can inhibit the inflanunation reaction,reduce the release of TF and attenuate the coagulation reaction.On the basis of
treatment with
low molecular weight heparin,application of the
tranexamic acid will not
lead to the formation of
thrombus,but the only use of the
tranexamic acid would have the contrary results.The rtsults of the study provide a theoretical and experimental basis for
clinical diagnosis and prevention of hypercoagulation induced by
injury and
infection.