Objective To investigate whether
fluoxetine has
therapeutic effect of clinical score and
brain derived neurotrophic factor (
BDNF) expression in
serum of
experimental autoimmune encephalomyelitis (EAE)model.
Methods Rats were randomly divided into
solvent control group (n=6) ,model
control group ( n= 10)and
fluoxetine group ( n= 10). The EAE model was prepared by injecting
guinea pig spinal cord homogenate subcutaneously. The clinical score was daily measured according to the sign and symptoms of
rats in the
behavior examination. The
serum BDNF level was measured by EL1SA. Results 1. Except for the
solvent control group,the first sign of EAE(
Piloerection) was detected on 4th day after
immunization of
rats from both model
control group and
fluoxetine group,then EAE
rats had distal
tail weakness on 8th day, and gradually developed into completely
tail paralysis and
limb paralysis. EAE
rats' clinical score reached the peak on 16th day after
immunization. 2. The clinical score of
fluoxetine group became scientifically lower than model group since 18th day after
immunization (
Fluoxetine group3.27 ± 0. 33; Model
control group4.66 ± 0. 55, P < 0. 05 ). 3. Compared with the model
control group,
fluoxetine did not significantly increase the expression of
serum BDNF in EAE model (
Fluoxetine group62.27 ± 0.43; Model
control group61.67 ± 0.85, P > 0.05 ). Conclusion
Fluoxetine reduced the clinical score of EAE since 18th day after
immunization,which indicates
fluoxetine could promote the recovery of neurological function in EAE
rats.
BDNF may not contribute to protective effect of
fluoxetine in EAE
animal.