Objective To explore the application of quantitative DNAanalysis in differential diagnosis of benign and malignant breast masses to aid the surgery plan formation.Methods Four hundred and eighty-eight patients with breast mass were enrolled into this study.Tissues of breast mass in patients were gained by fine-needle aspirationpuncture.Two sections were made from each sample,one was stained by Papanicolaou for regular cytologyanalysis and another was stained with Feulgen for quantitative DNAanalysis.Pathological results were confirmed in each case after surgery.Results One hundred and sixty-four cases were classified as patients with benign neoplasm,while the other 324 cases were classified as malignant neoplasm,according to the pathological examination results.The sensibility and specificity were 91.4%(296/324) and 92.7%(152/164) for regular cytological method,90.1%(292/324) and 100.0%(164/164) for quantitative DNAanalysismethod.Meanwhile the positive predictive and negtive value of quantitative DNAanalysis was 100.0%(292/292) and 83.7%(164/164),of which regular cytological methods were 96.1%(296/308) and 84.4%(152/180).Conclusion The quantitative DNAanalysis might assistant differential diagnosis of benign and malignant breast tumor.