Objective To
research Dengue virus type 2 (DENV-2) to
human umbilical vein endothelial cells (HUVEC)
permeability.
Methods The titration of DENV-2 was detected and HUVEC
infection DENV-2 layer of
cells. At different
time points after
infection (30 min, 1 h, 3 h, 6 h, 12 h, 30 h,36 h, 24 h, 42 h, 48 h,72 h), the
permeability in HUVEC were detected after
Dengue virus infection. The
virus load in HUVEC was detected by
quantitative real-time PCR. And
ultrastructure in HUVEC was observed by
electron microscope. Results The
permeability in HUVEC was changed after
Dengue virus infection by
time lasting. The most
permeability in HUVEC was changed after
Dengue virus infection at 30 min and 42 h. And at the same
time the
virus load were most value in all of
time. The results of the
cell membrane were changed by
electron microscope. The deciduous
cell microvillus and dissd endochylema were founded. And some of
nuclear membrane blank was wided by
Dengue virus. Even the
leakage was founded in
cell nucleus. The other change included that disappearance mitochondrial and vacuolization crista, elder pith change
mitochondria. In addition, the complete of
cell membrane were dissolved. Conclusion The
permeability of HUVEC was changed by DENV-2. To explore the pathogenesis of
Dengue virus provide certain theoretical basis.