Objective To investigate the
role of
Beclin 1 in the genesis and development of
osteosarcoma and the effect of
Beclin 1 overexpression on the
growth of the
in vitro osteosarcoma cell line MG63.
Methods Real
time-
polymerase chain reaction (RT-PCR) and
Western blot were used to detect the expressions of
Beclin 1 in MG63 and hFOB1.19 at
mRNA and
protein levels ; A eukaryotic
clone of
plasmid pEGFP/Beclinl fusion with
protein EGFP/
Beclin 1 was constructed and was transfected into
human osteosarcoma cell line MG63 by using lipofectamine 2000.The effect of
Beclin1 overexpressions on the proliferation of MG63
cells was evaluated by MTT assay.
Cell apoptosis was measured by flow cytomerty(FCM).Results The
mRNA and
protein expression of
Beclin1 in
human osteosarcoma cell line MG63 was significantly lower than that in the
human osteoblast cell line hFOB1.19(0.17 ±0.06 vs 0.43 ±0.11,t =29.493,P <0.01 ; 0.13 ±0.05 vs.0.25 ± 0.08,t =6.325,P < 0.01).The
transfection of pEGFP/Beclinl increased the
mRNA levels of
human osteosarcoma(5.34 ± 0.50) times in transfected
tumor cells MG63.The rate of
cell apoptosis was low in control or transfected with lipofectamine 2000 only
cells at an average of(0.10 ± 0.05) %.The
apoptosis rate was significantly higher in pEGFP/
Beclin1 transfected
cells than control
cells ((4.3 ± 0.8) %,t =5.752,P < 0.05).Conclusion Compared with control
cells,Bedin1 is down-regulated in the
human osteosarcoma cell line MG63,which indicate the
role of
Beclin 1 in regulating the malignant
behaviors of
osteosarcoma.
Beclin1 overexpressions inhibits
cell proliferation and induces
apoptosis in MG63
cells.