The
paper is to
report the study of the effect of Shenfu
injection on the
enzyme activity of
liver CYP450 and its
mRNA level of
rat liver.
Microsome of
rat liver was prepared after
intravenous administration of Shenfu
injection for 7 days. The
enzyme activity was quantified by Cocktail
method. Meanwhile, the
mRNA expression of
CYP1A2,
CYP2B1/2, CYP2C11 and CYP3A1 in the
liver was detected by RT-PCR. Shenfu
injection obviously induced the
enzyme activities of CYP2B and CYP2C. Meantime Shenfu
injection decreased the
enzyme activities of
CYP1A2 and
CYP3A. The
mRNA levels of CYP2B and CYP2C were also induced in
rats treated with Shenfu
injection. But it obviously inhibited the
mRNA level of
CYP1A2 and
CYP3A. Since the
enzyme activity and
mRNA level were obviously changed after
administration, the potential effect of
drug-
drug interaction should be concerned.