Objective
Platelet aggregation, activation induced by bubbles is the main cause of
decompression sick-ness.
Clopidogrel(Clo) can decrease
platelet aggregation through inhibiting the bind of
fibrinogen and
ADP .This study is designed to find if
Clopidogrel can paly a protective
role in
decompression sickness and explore the intervention mechanism .
Methods Totally 111
male SD
rats divided into 3 groupsnormal
control group (n=20),
decompression sickness(DCS) group(n=46), and DCS+Clo(
Clopidogrel)treated
decompression sickness (DCS+Clo)group(n=45).The
rats in DCS and DCS+Clo group were placed in chamber and compressed to 1.5 MPa at speed of 2t/4 , the
time of compression and res-idence was 4.5 min totally, then decompressed to surface at a speed of 3 m/s.The
mortality and behavioral of
rats were ob-served within 30 min post
decompression .The
pathology and the wet/dry ratio of
lung , WBC and
platelet counts in periph-eral
blood, the expression of activated
platelets , and immunohistochemical
detection of
lung tissue CD 41 expression were also been tested .Results We found Clo reduces the DCS
mortality risk ( mortality rate11/45 in DCS+Clo group vs 28/46 in DCS group, P<0.01).Clo reduced the
lung injury, the wet/dry ratio of
lung, the accumulation of
platelet and leu-kocyte in
lung , the WBC counts and activated
platelets in peripheral
blood .Conclusion Clo can
play a protective
role in
decompression sickness through reducing post-
decompression platelet consumption and activation , decreasing the activation of
leukocytes .