Objective To study the effect of
heat stress on the
permeability,
cytoskeleton and
cell cycle of
human skeletal muscle cell (HSKMC).
Methods The HSKMC
membrane permeability was detected by
calcium ion inflow with flow cytometer,the
cytoskeleton was stained by CBB 250,and the
cell cycle was determined by flow cytometer.Results After 1 h of
heat stress on
human HSKMC
cells under different
temperature gradient,the median level of
calcium ion was 91.63 in 43 ℃
heat stress group compared with 22.98 in 37 ℃
control group.As
temperature increased,thicker and shorter
cytoskeleton and
stress fiber were shown under the high
power lens of microscope.The
DNA expression of
skeleton cells at G0/G1 stage was 44.13-62.98 in groups under
heat stress.Compared with normal
control group,
DNA expression was much higher in
heat stress group,when HSKMC was cultured under 37 ℃
temperature for another 18 h,it kept decreasing
DNA expression to a
similar level as
control group.Conclusions
Heat stress can cause
calcium iron inflow resulting in intracellular
calcium overload,and
affect the
cytoskeleton leading to loss of normal web ordered arrangement and increased gap in HSKMC
cells,which give rise to blocking
cell cycle into G0/G1 stage.