The pIRES2-EGFP/MAGE-A3 eukaryotic expression plasmid has been transfected into B16 cells successfully, the green fluorescence of fusion protein expression was found, and MAGE-A3 mRNA transcription in B16 cells expressions were detected in positive clones.
Conclusion:
The pIRES2-EGFP/MAGE-A3 eukaryotic expression plasmid has been transfected effectively and expressed stably by liposomemethod in the B16 cells.The expression of MAGE-A3 tumorcell model has been successfully established,which provide data for the study of laryngocarcinoma immunotherapy.