Objective To establish a
rat model at the same
time in accordance with the “hot sheng
syndrome” of
traditional Chinese medicine and primary
immune thrombocytopenic purpura of peripheral
blood platelet reduction.
Methods Using
back multi-point
injection of 20% dry
yeast suspension on SD
rats and 1∶4
dilution of
rabbit anti SD
rats platelet serum (APS) by
intraperitoneal injection to establish a primary
immune thrombocytopenic purpura “
heat sheng”
rat model.And observing
rats of TCM
syndrome characteristics, hemogram, myelogram and
serotonin (
5-HT) level of the
temperature regulating center in
thalamus.Results After
injection of 2 h ~6 h
temperature and daily
water of the model group
rats increased significantly,
toe purper showed in fourth day of modeling and intestinal mucosal
bleeding in thirty day of modeling(P <0.05);
Platelet count in peripheral
blood decreased significantly,
bone marrow megakaryocyte number reduced significantly((P <0.05);
5-HT level of the
temperature regulating center of
brain increased significantly((P <0.05).Conclusions The study of the primary
immune thrombocytopenic purpura heat sheng
rat model of combination of
disease and
syndrome reflected basically the pathological characteristics of
purpura caused by “
heat sheng” in primary
immune thrombocytopenic purpura rat mode.