Objective To investigate the intervention of chemokine receptor 4(CXCR4) antagonist AMD3100 in lungtissues of rats during pulmonary oxygen intoxication.Methods Forty SD rats were randomly divided into 4 groupsnormal pressureair PBS group, normal pressureair antagonist group , oxygen exposure PBS group and oxygen exposure antagonist group, each consisting of 10 animals.The last two groups were compressed to 0.23 MPa at an exponential rate of 0.1 MPa/min by pure oxygen.Pathological changes of lungtissues were observed by hematoxylineosinstain.Changes in TNF-αand IL-1βexpression levels in the lungtissues of rats were detected by ELISA.Changes in CXCR4 expression levels were ob-served by Western blotting.Results Pathological examination indicated that edema and hemorrhage in the alveolar and pulmonary interstitial tissue of oxygen exposure antagonist group were lighter than in oxygen exposure PBS group.The levels of TNF-α, IL-1βand cleaved-caspase-3 in the lungtissues of the oxygen exposure antagonist group were lower than in oxy-gen exposure PBS group.Conclusion Blocking CXCR4 with AMD3100 can effectively alleviate lung injury during pulmo-nary oxygen intoxication.