Genomic and phylogenetic analysis of porcine mitochondrial genomes of Rongshui miniature pig / 中国比较医学杂志
Jiayue XU; Hehe SHI; Xumin WANG; Xiang GAO; Xiyong YU; Jun YU; Xiaojiang TANG.
Chinese Journal of Comparative Medicine
; (6): 28-34, 2015.
Artículo
en Zh
| WPRIM | ID: wpr-464563
Objective Assembly whole mitochondrial genome sequence of Rongshui miniature pig ( RMP ) breed and analysis the structure of mitochondrion based on the next-generation sequecing method.Comparison of phylogenetic relationship and genetic diversity among different pig breeds.Methods We collected peripheral venous blood sample from RMP and constructed two paired-end sequencing libraries.A whole-genome shotgun sequencing strategy and Illumina Genome Analyser sequencing technology were used in our study.Results The mitochondrial genome of RMP consists of 13 protein coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and the length of pig is 16888 bp.The GC content of this pig mitochondrial genome is about 44 %.Based on phlogenetic analysis, population genetic analysis, our findings confirmed that the ancestral cluster in East Asia mainly occurred among Diannan 7#pig, Hainan wild boar, Lanyu and RMP.Conclusion RMP, a typical miniature pig breed in China, is an earlier ancestor than Lanyu pig breed.
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