BACKGROUND:
MicroRNA plays an important
role in the process of
growth and
aging of living body. To know the
role of
let-7d in inducing
bone marrow mesenchymal
stem celldifferentiation into
neurons can promote the
stem celltransplantation.
OBJECTIVE:
To investigate the
role of
let-7d in inducing
bone marrow mesenchymal
stem celldifferentiation into
neurons.
METHODS:
(1) The lentiviral vector of
let-7d was constructed and transfected into
rat bone marrow mesenchymal stem cells. The
cells were divided into non-transfected group, negative
control group (transfected with FU-
RNAi-NC-LV), transfected enhancement group (transfected with
let-7d-LV), transfected inhibition group ( transfected with
let-7d-inhibition-LV). (2)
Rat bone marrow mesenchymal stem cells were treated with fasudil as an inducer for triggering the
cells to differentiate into
neurons. The expression of
neuron-specific markers,
neuron-specific enolase and
microtubule-associated protein 2, were measured by immunocytochemical
method. The
mRNA expression of
microtubule-associated protein 2 was detected by RT-PCR. The viability of
bone marrow mesenchymal stem cells was determined by MTT
method. RESULTS AND
CONCLUSION:
Under inverted
fluorescence microscope, the
cells were successful y transfected with
let-7d. Fasudil induced
bone marrow mesenchymal stem cells to differentiate into
neurons. The
transfection efficiency and expression levels of
neuron-specific enolase and
microtubule-associated protein 2 in transfected enhancement group were higher than those in the negative
control group (P<0.05);while in the inhibition group, they were lower than those in the negative
control group (P<0.05). These findings indicate that
let-7d can promote the differentiation of
bone marrow mesenchymal stem cells into
neurons induced by fasudil, and by control ing the expression of
let-7d we can influence the differentiation
efficiency from
bone marrow mesenchymal stem cells to
neurons.