ObjectiveTo study the effect of
heat stress on the
cytoskeleton and
cell cycle of
human umbilical vein endothelial cell (HUVEC)
in vitro.
Methods HUVEC was cultured
in vitro in 5%CO2 medium at 37℃ (
control group) or 43℃ (
heat stress group) for 1 hour. Coomassie brilliant blue R-250
staining was used to determine the effect of
heat stress on the
cytoskeleton. The
cells in
heat stress group were subsequently cultured at 37℃in 5%CO2 medium after
heat stress for 1 hour, and
cell cycle of HUVEC was determined at 0, 6, 12, 18 and 24 hours with
flow cytometry.Results Under
light microscopy normal
cytoskeleton was observed in
control group, but thicker and shorter
cytoskeleton was found after a rise of
temperature, and
stress fibers were found in
heat stress group. The
DNA content of HUVEC at all
time points in G0/G1 stage was 38.07%-55.19% after
heat stress. The
DNA content in
control group was 48.57%, and it was 54.06%, 55.19%, 48.23%, 38.07%, and 41.03% at 0, 6, 12, 18, 24 hours in G0/G1 stage in
heat stress group.
DNA content in
S phase was 35.33%-48.18%. The
DNA content in
control group was 44.62%, and it was 35.33%, 39.50%, 42.50%, 48.18%, and 47.99% at 0, 6, 12, 18, 24 hours in S stage in
heat stress group.
DNA content in G2/
M phase was 5.31%-13.75%. The
DNA content in
control group was 6.81, and it was 10.61%, 5.31%, 9.27%,13.75%, and 10.98% at 0, 6, 12, 18, 24 hours in G2/M stage in
heat stress group. It was demonstrated that compared with
control group, the
DNA content in G0/G1 stage was significantly increased when the HUVEC were
separated from
heat stress within 6 hours, and it recovered at a
similar level as
control group at 12 hours.Conclusion
Heat stress can change the
cytoskeleton of HUVEC, and cause stagnation at G0/G1 stage in
cell cycle.