Objective To observe the effects of
White Mange Mixture on the content of
VEGF and the genetic and
protein expressions of
VEGFR-2 of
HaCaT cells;To explore its mechanism.
Methods HaCaT cells were cultured
in vitro to establish
psoriasis experimental model. SD
rats were randomly divided into the blank
serum group,
white mange mixture of low-, medium- and high-
dose groups and
acitretin group, meanwhile to set up blank
control group. Cultured
HaCaT cells model were intervened with different
medicine serum.
ELISA was applied to detect the content of
VEGF. RT-PCR and
Western blot were used to detect the genetic and
protein expressions of
VEGFR-2.Results Compared with the blank
control group,
White Mange Mixture of low-, medium- and high-
dose groups interventing
HaCaT cells could obviously inhibit the
secretion of
VEGF and decrease the genetic and
protein expression of
VEGFR-2 in a
dose-dependent manner. Conclusion
White Mange Mixture
plays a
role in the
treatment of
psoriasis possibly through inhibiting the content of
VEGF and decreasing the genetic and
protein expressions of
VEGFR-2.