SUMMARY Thehumanembryonicstemcells(
hESCs)serveasaself-renewable,genetically-healthy, pluripotent and single source of all body
cells,
tissues and organs.Therefore,it is considered as the good standard for all
human stem cells by US,
Europe and international authorities.In this study,the standard and healthy
human mesenchymal progenitors,
ligament tissues,
cardiomyocytes,
keratinocytes,primary
neurons,
fibroblasts,and salivary serous
cells were differentiated from
hESCs.The
human cellular
health-
safety of NaF,
retinoic acid,
5-fluorouracil,
dexamethasone,
penicillin G,
adriamycin,
lead ace-tate PbAc,bisphenol A-biglycidyl
methacrylate (
Bis-GMA)were evaluated selectively on the standar-dized platforms of
hESCs,
hESCs-derived
cardiomyocytes,
keratinocytes,primary
neurons,and fibro-blasts.The evaluations were compared with those on the currently most adopted cellular platforms.Parti-cularly,the
sensitivity difference of PM2.5
toxicity on standardized and healthy
hESCs derived
fibroblasts, currently adopted immortalized
human bronchial
epithelial cells Beas-2B and
human umbilical vein endo-thelial
cells (HUVECs)were evaluated.The results showed that the standardized
hESCs cellular plat-forms provided more
sensitivity and accuracy for
human cellular
health-
safety evaluation.