Enhancement of γδT cells proliferation and cytotoxicity by Hyperoside / 中国免疫学杂志
Ying LI; Yu ZHOU; Leiqing SUN; Zhonghai ZHOU; Xiaoting Lü; Ming XU; Yi LI; Junquan LIU.
Chinese Journal of Immunology
; (12): 524-527, 2016.
Artículo
en Zh
| WPRIM | ID: wpr-486001
Objective:
To investigate the anti-tumor effect of Hyperoside.Methods:
Human γδT cells were amplified by isopentenyl pyrophosphate from peripheral blood cells.The proliferation capacity of γδT cells was measured with CCK-8 assay after treated with different concentrations of Hyperoside.Cytotoxicity of γδT cells was detected with LDH assay , and the expression of granzyme,perforin CD107a and IFN-γonγδT cells were measured by flow cytometry before and after treatment.Results:
Hyperoside could significantly stimulate the proliferation of γδT cells at the concentration of 3.13-12.5 μg/ml.Cytotoxicity and expression of granzyme,perforin and IFN-γofγδT cells were increased after treatment.Conclusion:
Hyperoside could enhance cytotoxicity of humanγδT cells through up-regulation of granzyme ,perforin CD107 a and IFN-γexpression.
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WPRO
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