Objective To investigate the expression of vascular endothelial factor C(
VEGF-C) and
E-cadherin in
gastric cancer and explore the relationship between expression of
VEGF-C and
E-cadherin and the pathogenesis of
gastric cancer.
Methods Real-
time quantitative reverse ranscriptase-polymerase chain rection was applied on 40 cases of
gastric cancer and corresponding adjacent noncancerous
tissues,in order to detect
mRNA expression of
VEGF-C and
E-cadherin gene.
VEGF-C and
E-cadherin protein expression in
gastric cancer and adjacent normal
gastric mucosa were detected by
immunohistochemistry.
Statistical analysis was carried out to analyze the correlation among
VEGF-C,
E-cadherin and
protein expression with various clinical
parameters in these
gastric cancer patients.Results The expression of
VEGF-C protein in 40 cases of
gastric cancer's
cancer tissues was 0.461±0.012,significantly higher than that in the adjacent normal
tissues(0.036+0.023;t=1.101,P<0.05),but
E-cadherin expression was significantly lower than that of the adjacent normal
tissues (0.079±0.002 vs.0.321±0.005;t=1.844,P<0.05).There was correlation between
VEGF-C mRNA expression with histological grading,
TNM staging,
lymph node and distant
metastasis (t=-1.621,-1.474,-2.378,-1.966,P<0.05).There was correlation between
E-cadherin mRNA expression with
tumor size,histological grading,TNM stage,there was a significant difference (t=1.875,1.673,1.544,P<0.05).
VEGF-C and
E-cadherin protein expression was negatively correlated(r=-0.688,P<0.05).Conclusion Up-regulated of
VEGF-C gene and decreased expression of
E-cadherin may
play an important
role in the
carcinogenesis of
gastric cancer