Objective To investigate the
association between
single nucleotide polymorphism (
SNPs) (rs1053005 and rs744166) and expression level of STAT3
gene and the susceptibility to
acute lymphoblastic leukemia (ALL) in
Chinese children.
Methods A
case-control study was performed,and 184
children with ALL and 377 healthy
children as controls were recruited.The
genotypes of 2
SNPs were detected by using
polymerase chain reaction-
restriction fragment length polymorphism method.And the expression level of STAT3
gene was detected by using
real-time PCR;All the data were analyzed by using SPSS 16.0
software.Results (1) In this study,the
genotypes (GG,AG,AA) of rs1053005 had a significant difference between the ALL group and
control group (x2 =6.737,P =0.034).Compared with
control group,the A
allele had a higher frequency in ALL group and A
allele was a
risk factor(x2 =5.853,P =0.016).But,there was no difference in frequency of
genotype rs744166 between the 2 groups (x2 =1.866,P =0.393).(2) There was no significant
association between
genotypes and
risk degree among 3 groups (high
risk group,medial
risk group and standard
risk group) (x2 =0.335,P =0.987).(3) The expression level of STAT3
gene in
patients with AA
genotype was lower than that of the
patients with GG
genotype (t =4.758,P =0.009);and compared with
patients of the standard
risk group,high
risk patients had a lower expression level of STAT3
gene (t =5.284,P =0.007).Conclusions The polymorphism of SNP rs1053005 was associated with ALL,with A
allele being a
risk factor;and the expression level of STAT3
gene maybe associated with the
risk degree in ALL
patients.