Objective To investigate the effects of Abnormal Phlegmatic Munziq on
ability of
learning and
memory , and
protein expressions of
brain tissue RAGE and LRP1 of APP/PS1 transgenetic
mice model of AD;To discuss its mechanism of action.
Methods Three-month-old APP/PS1
transgenic mice were randomly divided into 5 groups model
control group , positive
control group , Abnormal Phlegmatic Munziq high-, medium-, and low-
dose groups, 18
mice in each group. Another 18 three-month-old C57BL/6J
mice were chosen as normal
control group . All
administration groups received relevant
medicine for successive 6 months. Then the changes in
ability of
learning and
memory of
mice were detected by Step-down test;
protein expressions of LRP1 and
RAGE were detected by
immunohistochemistry and
Western blot . Results Compared with the normal
control group , the
reaction time of
learning grades and the mistake times increased, incubation of
memory grades decreased and the mistake times increased in the model
control group (P<0.01);Compared with the model
control group , the
reaction time of
learning grades and the mistake times decreased, incubation of
memory grades increased and the mistake times decreased in all
administration groups (P<0.05, P<0.01).
Immunohistochemistry and
Western blot results showed that compared with normal
control group , the LRP1 expression decreased and
RAGE increased in the model
control group (P<0.05);Compared with the model
control group , the LRP1 expression decreased and
RAGE increased in Abnormal Phlegmatic Munziq high-, medium-, and low-
dose groups (P<0.05,P<0.01). Conclusion Abnormal Phlegmatic Munziq can improve
ability of
spatial learning and
memory in APP/PS1
mice and regulate the expressions of
RAGE and LRP1.