CD44v6 was known as
tumor marker for
tumor progression and
metastasis in various kinds of
carcinomas. The CD44v6
monoclonal antibody was produced by
cell cultures or
mouse ascite fluids using CD44v6
hybridoma cells, and its immunogloburin G (
IgG) was purified by
Protein A column. Using immobilized
ficin and
cysteine, the
antibody fragment Fab was produced and purified by
Protein A. Four CD44v6 scFv molecules were produced from the
recombinant DNA and
phage antibody
technology and prurified by His-tag
affinity chromatography. In order to inspect the function and
specificity of each antibody molecule, western-blotting and
ELISA against CD44v5-6
recombinant proteins and irnmunodetection in
human ovarian
carcinomas were estabilished. The results showed that
immunodiagnosis did not distinguish the types of
antibody fragments, but western-blotting and
ELISA results did show some difference of their specificities and
biological properties. These studies
will contribute as a model study for the
immunodiagnosis and
therapy using the
IgG, Fab and scFv of CD44v6 antibody to obtain the early
detection of
tumor progression and
metastasis using immunoscintigraphy.