Objective To determine the effects of
enflurane and
isoflurane on the spontaneous neural discharge of
central amygdaloid nucleus in
rats Methods SD
rats (30 60 d) of either
sex were decapitated
Brain was immediately removed and kept in 4℃ artificial cerebral spinal fluid(ACSF) which was balanced with 95% O 2 and 5% CO 2 gas mixture Braine
tissue containing central amyfdaloid nucleus was cut into slices of 300 400?m thick which were immersed in ACSF
Enflurane and
isoflurane were administered by balancing ACSF with
enflurane (1 5%,3 0%,4 5%) or
isoflurane (1 1%,2 2%,3 3%) The spontaneous neural discharge of
central amygdaloid nucleus was measured before and after
enflurane or
isoflurane using whole
cell patch clamp techniques Results
Enflurane and
isoflurane inhibited the frequencies of spontaneous neural discharge of
central amygdaloid nucleus in a
dose dependent manner The spontaneous neural discharge inhibited by
enflurane (3 0%) and
isoflurane (2 2%) could recover after the slices being washed with normal ACSF for 5 min Conclusions The results indicate that the spontaneous neural discharge of
central amygdaloid nucleus can be inhibited reversibly by
enflurane and
isoflurane Central amygdaloid nucleus may by one of the sites of action of
enflurane and
isoflurane in
central nervous system