AIM:
To investigate the influence of Sini decoction (SND) on the proliferation and
apoptosis of
rabbit abdominal aorta smooth muscle cells after ballon
injury and discuss the effect of
vascular smooth muscle cell's (VSMCs) proliferation and
apoptosis in post-
percutaneous coronary intervention (PCI) restenosis (RS) and the feasibility of SND preventing post-PCI RS.
METHODS:
The
animal model of
rabbit abdominal aorta ballon
injury was set up and the therapertic group was treated with SND. The shape of proliferative and apoptotic
cell were investigated by
electron microscope.
Immunohistochemistry staining was performed using ?-
actin,
PCNA and
Cyclin E monoclonal antibodies. In situ
Cell Death Detection Kit was used to identify apoptotic
cells. Abdomial
aorta angiography was operated in the 84th day subgroup and the
stenosis degree was evalued by quantitative angiographic
analysis.
RESULTS:
As compared with the
control group, the
therapeutic group displayed a lower proliferative percentage and a higher apoptosic percentage (P