Nucleostemin specific RNAi influences cell proliferation in HeLa cells in vitro and in vivo / 中国病理生理杂志
Ziwei CAI; Sijin LIU; Liqiu SUN; Weide LAO.
Chinese Journal of Pathophysiology
; (12)2000.
Artículo
en Zh
| WPRIM | ID: wpr-525363
AIM:
To examine Nucleostemin (NS) expression in tumor cells, and observe the effect of NS specific RNA interference on the cell proliferation in Hela cells.METHODS:
Total RNA was extracted from 6 kinds of cultured tumor lines, the NS expression level was measured by RT-PCR and Northern blot. An NS-specific siRNA expression vector was constructed to transfect HeLa cell (NS-siRNA-HeLa), and the proliferation of the cell was observed.RESULTS:
NS was highly expressed in 6 kinds of tumor cells. NS expression level in the NS-siRNA-HeLa cells was remarkably reduced, and the percentage of G_0/G_1 cells increased. The neoplasm forming ability in nude mice by the NS-siRNA-HeLa cells was decreased.CONCLUSION:
NS is highly expressed among tumor cells. NS-specific siRNA inhibits the entry of the cell cycle into the S phase, and remarkably reduces the proliferation ability of HeLa cells in vitro and in vivo.
Biblioteca responsable:
WPRO
Texto completo
Documentos relacionados