Effects of crocetin on the apoptosis and the changes of its related regulating proteins caspase-3 and Bcl-2 induced by H_2O_2 in myocardial cells / 中国病理生理杂志
Weiping YU; Guanglin XU; Chengxing SHEN; Zhiyu QIAN.
Chinese Journal of Pathophysiology
; (12)1986.
Artículo
en Zh
| WPRIM | ID: wpr-526555
AIM:
To observe the effect of crocetin on the apoptosis and the changes of its related regulating proteins caspase-3 and Bcl-2 expression induced by hydrogen peroxide (H_2O_2) in cultured cardiomyocytes.METHODS:
Changes of cellular morphology were detected under microscope. Apoptosis rates of the cells were analyzed by PI staining with flow cytometry. Expressions of caspase-3 and Bcl-2 proteins in the cells were determined by immunofluorescence with flow cytometry.RESULTS:
In the concentrations used, more severe morphological changes with higher apoptosis rate of the cultured myocardial cells were seen in each H_2O_2 group than that in control group. When treated with 1?10 -4 mol?L -1 H_2O_2, the caspase-3 was increased and Bcl-2 protein decreased remarkably in the cells. But each dosage of crocetin, especially the highest one (5?10 -5 mol?L -1, P
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