To extract and separate the water-soluble chemical constitutions from Radix Isatidis.METH ODSThe Radix Isatidis water extract underwent adsorption by D 101 macroporous resin,the portion eluted by ethanol of different concentrations was isolated and purified on silica gel column repeatedly,the physicochemical constants and the spectra data of the compound obtained were determined and its chemical constitutions were identified.
RESULTS:
3compounds temporarily separated from Radix Isatidis water extract,were identified assyringin(Ⅰ),indole—3—acetonitrile—6—O—?—D—glucopyranoside(Ⅱ)and(+)—isolariciresinol(Ⅲ),respectively.
CONCLUSION:
For the first time,compound(Ⅰ)and compound(Ⅱ)were isolated from Radix Isatidis.