Objective To systematically assess the
detection of
K-ras gene mutation at
codon 12 in
pancreatic juice for
diagnosis of
pancreatic cancer.
Methods A comprehensive
electronic searching was performed to retrieve relevant studies on
K-ras gene mutation in
pancreatic juice for
diagnosis of
pancreatic cancer.Data on accuracy of included studies were extracted,Meta-DiSc1.4,Stata10.0,was applied for further heterogeneity exploring,
meta-analysis and
publication bias testing.Results Fifteen studies met the inclusion criteria.Heterogeneity was not found among these studies,including threshold effect,different test
methods,
randomization and blind.Pooled accuracy
indicators like
sensitivity,
specificity and diagnostic
odds ratio(DOR) were 0.61(95%CI 0.56-0.66),0.82(95%CI 0.77-0.86) and 6.28(95%CI 4.42-8.91),respectively.
Area under curve(
AUC) of SROC(summary
receiver operating characteristics) was 0.8207 and Q index was 0.7542.
Publication bias had little influence on
meta-analysis with the fail-safe number of 694.0108.Conclusions This
meta-analysis demonstrates that the
detection of
K-ras gene mutation in
pancreatic juice has moderate effect for
diagnosis of
pancreatic cancer.It is not enough to be an early diagnosing or
screening indicator for
pancreatic cancer,but it can be used clinically as an important adjunct to
cytology,imageology and
enzymology in the
diagnosis of
pancreatic cancer.