Objective:
To establish a metastatic
cell line from distant organ
metastasis using Mc3
cell line in
nude mice.
Methods:
Tail vein injection of Mc3
cells and
cell culture technic were employed to induce
metastasis in distant organ .
Cell counting and
flow cytometry were used to study the
cell growth.
Karyotype analysis and histopathological
observation were used to study the morphological features with
light and
electron microscopy.
Results:
Paralized
nude mouse was observed in 1 out of 50 experimental
nude mice. The
cells derived from the
spinal cord were cultured and transferred for more than 50 passages. The
cells were proved to be of
mucoepidermoid carcinoma from
human being by the morphology, histopathology and
karyotype of the
cells. The
population doubling
time and S-phase
cell of the
cells were 43 h and 22.7% respectively. The
cell line was named Ms.
Conclusion:
Ms is a metastatic
cell line of
spinal cord metastasis in
nude mouse derived from
human mucoepidermoid cacinoma
cells.