Background and
purpose:
Gallbladder carcinoma, lacks of specific clinical manifestations,and is usually diagnosed at advanced stages of the
diseases and even fewer can be resected.
Chemotherapy has not shown significant activity in
gallbladder carcinoma,so new agents should be applied to its
treatment.
Arsenic trioxide,verified as a breakthrough in the management of
acute promyelocytic leukemia,has been applied in the
research of a variety of solid
tumors.This study is to investigate the
biological effect of As_(2)O_(3) on
human gallbladder carcinoma cell GBC
cells and its mechanism.
Methods:
Hoechest33258
staining,
DNA gel
electrophoresis and flowcytometric were used to determine
apoptosis.
Western blot was performed to
analysis apoptotic
proteins expression.
Results:
The
treatment of As_(2)O_(3) in
gallbladder carcinoma cells could induce
apoptosis in a
dose-dependent manner and downregulate the expression of
anti-apoptotic protein Bcl-2.Moreover,Bcl-2 overexpression could protect
gallbladder carcinoma cells from As_(2)O_(3)-induced
apoptosis.
Conclusions:
As_(2)O_(3) induces
gallbladder carcinoma cell apoptosis via
down regulation of Bcl-2.