50 g/ml by MIC. Nine isolates contained the qacA/B, and 1 isolate contained the smr gene. No qacG,qacH and qacE?1 was detected. Conclusion The disinfect resistance of Staphylococcu spp isolated from hospitaldisinfectantenvironment is markedly high. Resistance monitoring should be strengthened,and the rational use of disinfect should be emphasized.