A
monoclonal antibody 1C34-5 raised against
human peripheral mononuclear
cells was produced. This antibody, a monoclonal
IgG1. showed positive reaction with about 90% of T and
B lymphocytes,
monocytes.
granulocytes and
bone marrow cells, but negative reaction with
red blood cells and
platelets by indirect immunoflurorescent
technique. 1C34-5 also reacted with leucocytic
cell lines except a non-T non-B lymphoid line Reh but not with erythroid line K562,
HeLa cells and
fibroblast cells so far tested.This antibody was also assayed histologically by an indirect
immunoperoxidase technique against a variety of
human normal
tissue frozen sections. It was found that 1C34-5 bound to all
lymphoid tissues including
lymph nodes,
tonsil,
thymus.
Peyer's patches and leucocytes scattered in other
tissues, but not to all non-hematopoietic
tissues as well as erythropoietic foci in fetal
liver. Thus. 1C34-5 appears to recognize a
human leucocyte
antigen specifically.