Objective To isolate and identify the putative
Japanese encephalitis virus (JEV) re-ceptors from C6/36 and
Vero cells.
Methods Molecules binding with JEV were isolated from C6/36 and
Vero cells by
co-immunoprecipitation (Co-IP) approach, identified by
mass spectrometry, and detected by
Western blot. The
location of putative JEV receptor on
cells membrane and the binding with JEV were ob-served by
laser scanning confocal microscopy (LCM). Results Several molecules binding with JEV were isolated from C6/36 and
Vero cells by Co-IP, and only one molecule was identified as
heat shock cognate 70 (HSC70) by
mass spectrometry. Antibody against HSC'70 was able to detect a 74 ×103
protein isolated by Co-IP from C6/36 and
Vero cells membrane in
Western blot assays. It was observed by LSCM that when JEV attached on the surface of C6/36
cells, JEV and HSCT0
protein were co-
localization. Conclusion 74 x 103 molecular identified as
HSC70 protein from C6/36
cells may be JEV receptor.