[Objective] To investigate the possibility of [
poly(lactic-co-glycolic acid) ,PLGA]
nanoparticles (NP) as a new kind of
protein drug carrier for
nasal administration . [
Method ] CNT-PLGA-NP was prepared by double emulsification
solvent evaporation . Its morphology was examined by
transmission electronmicroscope (TEM). In the use of photon correlation
spectroscopy (PCS) and
laser Doppler anemometry(LDA),its
Zeta potential and mean
particle size were estimated .Also, the entrapment
efficiency of CNT-PLGA-NP and its
pharmacokinetics in
rats through
nasal administration were studied. [Result] The
Zeta potential , mean
particle size , entrapment
efficiency of CNT-PLGA-NP were-13.4mV, 320.20nm and 45% respectively .Its vitro CNT release from CNT-PLGA-NP appeared to consisting of two components with initial rapid release followed by a exponential stage.The
AUC and t1/2(?) of CNT, CNT-PLGA-Np and CNT-PLGA-NP(B+T)were 1.14, 8.37, 7.12?g?h/mL and 20.06, 44.14, 34.82h respectively. [Conclusion]PLGA-NP might be a potential new
drug carrier for CNT.
Polysorbate 80 and borneol might be good
absorption enhancers.