Objective To observe the effect of
peroxisome proliferators activated receptor gamma(
PPAR-?)on phenotypic transforming of
vascular smooth muscle cells(VSMC)in
hypertension.
Methods Spontaneous
hypertension rats(SHR)and
WKY rats both
aged 4 months were included.
SHR rats as well as
WKY rats were divided to be fed with normal chow,and chow added with
rosiglitazone(10 mg?kg-1?d-1)for 16 weeks.VSMC were isolated from
SHR rats and
WKY rats and cultured by patch-attaching
method,then respectively divided into 3 groups after treated with
genetic recombination technologynormal VSMC,
PPAR? overexpressed VSMC and
PPAR? silenced VSMC.Expressions of OPN and ?-SMA,which respectively represent the undifferentiated and differentiated VSMC,were detected by
Western blotting.
Cell proliferation was determined by detecting
DNA synthesis and
cell counting.The changes of
arteries were evaluated pathologically.Results
Rosiglitazone decreased
blood pressure and ameliorated
vascular remodeling of
aorta in
SHR rats.
Aorta of SHR showed an
upregulation of OPN and
downregulation of ?-SMA,which could be inhibited by
rosiglitazone.VSMC from
SHR rats showed an
upregulation of OPN and
downregulation of ?-SMA,and increased
cell proliferation.These changes were all inhibited by
rosiglitazone.In the
cells that overexpressed
PPAR?,the
cell proliferation rate was lower,and the expressions of OPN and ?-SMA were depressed,compared with the corresponding control
cells.Conclusion
PPAR-? could inhibit the phenotypic transforming of VSMC,and this might be responsible for the amelioration of
vascular remodeling in
hypertension.