Aim To investigate the effects of
Astragalus Mongholicus Bunge
Lectin (AMML) on
tumor cells proliferation,
cell cycle and
apoptosis by using
human leukemia cell line (
K562 cells).
Methods The antiproliferation effect of AMML on
K562 cells was detected by the colorimetric MTT assay.The
apoptosis induced by AMML on
K562 cells was explored by means of
cell morphological and
flow cytometry.Results AMML showed strong inhibiton of the
growth of
K562 cells in a
time-and concentration-dependence. After incubation of
K562 cells with AMML at a concentration of 60 mg?L-1 for 72 h,the inhibition ratio was 89%.Morphological
observation showed that AMML-treated
K562 cells displayed outstanding
apoptosis characteristics,such as nuclear fragmentation,
chromatin condensation. AMML induced significant
cell cycle arrest at
S phase in
K562 cells,and the
apoptosis of
K562 cells was confirmed by
flow cytometry.Conclusion AMML can inhibit the
growth of
K562 cells through S arrest and induce the
apoptosis of
K562 cells. Thus,AMML may be valuable for the
treatment of
cancer.